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KMID : 0545120110210101036
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 10 p.1036 ~ p.1042
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Chemical Modification of Botryosphaeran: Structural Characterization and Anticoagulant Activity of a Water-Soluble Sulfonated (1→3)(1→6)-β-D-Glucan
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Jamile Brandi
Eder C. Oliveira
Nilson K. Monteiro
Ana Flora D. Vasconcelos
Robert F.H. Dekker
Aneli M. Barbosa
Joana L. M. Silveira
Paulo A. S. Mourao
Maria de Lourdes Corradi da Silva
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Abstract
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The exopolysaccharide botryosphaeran (EPSGLC; a (1→ 3)(1→6)-β-D-glucan from Botryosphaeria rhodina MAMB- 05) was sulfonated to produce a water-soluble fraction (EPSGLC-S) using pyridine and chlorosulfonic acid in formamid. This procedure was then repeated twice to produce another fraction (EPSGLC-RS) with a higher degree of substitution (DS, 1.64). The purity of each botryosphaeran sample (unsulfonated and sulfonated) was assessed by gel filtration chromatography (Sepharose CL-4B), where each polysaccharide was eluted as a single symmetrical peak. The structures of the sulfonated and re-sulfonated botryosphaerans were investigated using ultraviolet-visible (UV-Vis), Fourier-transform infrared (FT-IR), and 13C nuclear magnetic resonance (13C NMR) spectroscopies. EPSGLC and EPSGLC-RS were also assayed for anticoagulation activity, and EPSGLC-RS was identified as an anticoagulant.
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KEYWORD
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botryosphaeran, anticoagulant activity, sulfonation, Botryosphaeria rhodina MAMB-05
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